Journal: iScience

Article Title: Bromodomain protein BRD8 regulates cell cycle progression in colorectal cancer cells through a TIP60-independent regulation of the pre-RC complex

doi: 10.1016/j.isci.2023.106563

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Article Snippet: pCAGGS-HA BRD8 (isoform 1: GenBank: NM_006696.4 , isoform 2: GenBank: NM_139199.2 ) and pcDNA3.1-Myc/His MRGBP (GenBank: NM_018270.6 ) were constructed as described previously. pcDNA4-TO-HA-Brd4FL was obtained from Addgene (#31351, Cambridge, MA, USA).

Techniques: Recombinant, Plasmid Preparation, Cell Cycle Assay, CCK-8 Assay, Flow Cytometry, Viability Assay, Ubiquitin Proteomics, Expressing, Cloning, Mutagenesis, Software

Journal: Autophagy

Article Title: FAIM regulates autophagy through glutaminolysis in lung adenocarcinoma

doi: 10.1080/15548627.2021.1987672

Figure Lengend Snippet: FAIM is overexpressed in lung adenocarcinoma and correlates with poor survival of lung adenocarcinoma patients. (A) The mRNA level of FAIM isoforms in lung adenocarcinoma cell lines (A549, H1299, H292, SPC-A1, H358) were determined by Q-PCR using specific primers. Data represent the average of three independent experiments (mean ± SD). ***, P < 0.001. (B) The protein expression of FAIM in lung adenocarcinoma cell lines (H1299, SPC-A1, H292, PC9, H1975, HCC827, H358, A549) and human bronchial epithelial cells (HBE) were determined by western blot with the indicated antibodies. (C) FAIM expression in tumor tissues (T) and adjacent normal tissues (N) from 18 NSCLC patients were determined by western blot with the indicated antibodies. (D) Immunohistochemical staining of a representative lung adenocarcinoma tissue microarray with FAIM antibody. T, tumor tissue; N, adjacent normal tissue. (E) Quantification of the immunohistochemical (IHC) staining shown in Figure 1D. (F) Microscopy evaluation of IHC staining of two representative tumor tissues and adjacent normal tissues shown in Figure 1D with FAIM antibody (brown) and hematoxylin counterstain (blue). Scale bars: 100 μm. (G) Kaplan-Meier survival curve of 90 lung adenocarcinoma patients. Patients were divided into two groups according to the average staining density of FAIM in cancer tissues of the tissue array (High expression: n = 42, low expression: n = 48, Log-rank (Mantel-Cox) test was used for the statistical analysis).

Article Snippet: The FAIM antibody was purchased from Proteintech (13005-1-AP).

Techniques: Expressing, Western Blot, Immunohistochemical staining, Staining, Microarray, Immunohistochemistry, Microscopy